| Analysis Note | Single band by SDS-PAGE. |
| Preparation Note | Prepared by a modification of the method of Haque, et al. |
| Reconstitution | When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl. |
| Biochem/physiol Actions | Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm. |
| General description | Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min. |
